A simple and sensitive HPTLC method has been developed for determination of Amlodipine besylate and Clopidogrel bisulphate in its pharmaceutical dosage forms. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. Hexane: Acetone: TEA (6.5:3:0.5 v/v) as a mobile phase. After that saturation time is optimized for the better separation The Rf value of Amlodipine besylate is 0.23±0.02 and of Clopidogrel bisulphate is 0.69±0.02 were found. The linearity for both the drugs were in the range of 50-350ng/band and 750-5250 ng/band. The % recoveries of Amlodipine besulate and Clopidogrel bisulphate were found to be in the range of 100.24-100.37 and 99.36-100.82 respectively. The proposed method was validated and successfully applied to the estimation of Amlodipine besylate and Clopidogrel bisulphate in combined dosage form.
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